Cognitive & NootropicUse Caution

Dihexa

Also known as: N-hexanoic-Tyr-Ile-(6) aminohexanoic amide, PNB-0408

An extremely potent cognitive-enhancing peptide derived from Angiotensin IV, reported to be 10 million times more potent than BDNF at improving synaptic connectivity and cognitive function.

Half-Life

Unknown (limited data)

Typical Dose

10-40 mg

Frequency

Once daily

Routes

Oral

Overview

Dihexa is a small peptide (technically an oligopeptide) that was developed at Washington State University by Dr. Joseph Harding and colleagues. It is derived from Angiotensin IV and was designed to improve cognitive function by enhancing synaptic connectivity.

The most striking claim about Dihexa is its extraordinary potency: researchers reported it is 10 million times more potent than BDNF at promoting synaptic connections, making it one of the most potent cognitive enhancers ever discovered.

Key Characteristics

  • Origin: Synthetic derivative of Angiotensin IV
  • Classification: Nootropic/neurotrophic peptide
  • Structure: Modified hexapeptide fragment
  • Target: HGF/c-Met receptor system
  • Unique Feature: Extreme potency at picomolar concentrations

Important Context

Research Status Warning: Dihexa has LIMITED human safety data. While preclinical results are impressive, it remains an experimental compound. The potency claims require careful interpretation, and long-term effects are unknown.

Mechanism

Primary Mechanisms

1. HGF/c-Met System Activation

Dihexa's primary target:

  • Activates Hepatocyte Growth Factor (HGF) receptor (c-Met)
  • HGF/c-Met signaling promotes neuronal survival
  • Enhances synaptic plasticity
  • Supports neurogenesis
  • Protects against neurodegeneration

2. Synaptogenesis Enhancement

Creating new synaptic connections:

  • Promotes dendritic spine formation
  • Enhances synaptic density
  • Improves neural network connectivity
  • Supports learning and memory

3. Neurotrophic Actions

Supporting brain health:

  • BDNF-like effects (but more potent)
  • Neuronal protection
  • Enhanced neural repair
  • Anti-apoptotic effects in neurons

4. Angiotensin IV Pathway

Derived from angiotensin system:

  • Related to AT4 receptor activation
  • Cognitive enhancement independent of blood pressure
  • Brain-specific effects
  • Memory-enhancing pathway

The Potency Claim Explained

"10 million times more potent than BDNF":

  • This refers to effective concentration (EC50)
  • BDNF works at nanomolar concentrations
  • Dihexa works at picomolar (10⁻¹² M) concentrations
  • Doesn't mean 10 million times the effect
  • Means same effect at 10 million times lower dose

Research

Research Limitations: Most Dihexa research is preclinical (animal studies). Human clinical trial data is very limited. Claims should be viewed with appropriate scientific skepticism.

Animal Studies

Cognitive Enhancement

Rodent studies showed:

  • Improved spatial learning (Morris water maze)
  • Enhanced memory consolidation
  • Better cognitive performance in aged animals
  • Reversal of scopolamine-induced deficits

Dementia Models

In animal models of cognitive impairment:

  • Restored cognitive function
  • Improved synaptic connectivity
  • Neuroprotective effects
  • Potential for dementia treatment research

Mechanism Studies

Synapse Formation

Laboratory research demonstrated:

  • Increased dendritic spine density
  • Enhanced synaptogenesis
  • Improved neural connectivity
  • Active at extremely low concentrations

HGF/c-Met Confirmation

Studies confirmed:

  • Activity dependent on HGF/c-Met
  • Blocked by c-Met inhibitors
  • Not working through other pathways
  • Specific mechanism of action

Human Experience

Anecdotal Reports

Limited human data includes:

  • User reports of improved cognition
  • Enhanced focus and clarity
  • Better memory recall
  • Variable individual responses

No Clinical Trials

As of 2026:

  • No published human clinical trials
  • No dose-finding studies in humans
  • Long-term safety unknown
  • Optimal dosing not established

Dosing

Experimental Compound Warning: Dihexa has NO established human dosing from clinical trials. All dosing information is from preclinical research extrapolation and user reports. Use extreme caution. This compound is highly experimental.

Research Protocols (Extrapolated)

ProtocolDoseFrequencyDuration
Conservative Start5-10 mgOnce dailyTest response
Commonly Reported10-20 mgOnce daily4-8 weeks
Higher Range (Reported)20-40 mgOnce daily4-8 weeks
Sublingual10-20 mgOnce daily4-8 weeks

Administration Notes

Oral

  • Can be taken orally (capsules or powder)
  • Bioavailability unknown
  • May be taken with or without food

Sublingual

  • Held under tongue for absorption
  • May improve bioavailability
  • Common user-reported route

Intranasal

  • Direct CNS access theoretical advantage
  • Dosing less established
  • Some user reports

Subcutaneous

  • Alternative injection route
  • Most consistent absorption
  • Less common

Cycling

Given unknown long-term effects:

  • Short cycles (4-8 weeks) commonly used
  • Breaks between cycles
  • Monitor for any adverse effects
  • Conservative approach recommended

Safety

Known Side Effects

Reported (Limited Data)

  • Generally well-tolerated in reports
  • Headache (some users)
  • Vivid dreams (reported)
  • Possible mood effects

Theoretical Concerns

HGF/c-Met Activation

  • c-Met is an oncogene
  • Involved in cancer cell growth/spread
  • Long-term activation effects unknown
  • Theoretical cancer risk concern

Unknown Long-Term Effects

  • No long-term human studies
  • Brain chemistry changes uncertain
  • Dependency/tolerance unknown
  • Withdrawal effects unknown

Contraindications

Avoid if:

  • History of any cancer
  • Family history of cancer
  • Currently taking cancer medications
  • Pregnant or breastfeeding
  • Under 25 (brain still developing)
  • Any serious medical condition

Cancer Risk Warning: Dihexa activates c-Met, a known oncogene involved in cancer progression. While short-term cognitive benefits may exist, the long-term cancer implications of chronic c-Met activation are unknown and potentially concerning.

Drug Interactions

  • Unknown (not studied)
  • Caution with any CNS-active medications
  • Caution with cancer treatments
  • Full interaction profile not established

Monitoring

Before Use

  • Understand experimental nature
  • Consider cancer risk assessment
  • Baseline cognitive testing (optional)
  • Discuss with healthcare provider

During Use

  • Track cognitive effects
  • Monitor for any adverse effects
  • Keep duration limited
  • Document experience

After Use

  • Note any persistent effects
  • Watch for withdrawal effects
  • Long-term monitoring prudent

Comparison to Other Nootropics

| Feature | Dihexa | Semax | Selank | |---------|--------|-------|--------| | Mechanism | HGF/c-Met | ACTH analog | Tuftsin analog | | Potency | Extreme | Moderate | Moderate | | Human Data | Very Limited | Extensive (Russia) | Extensive (Russia) | | Cancer Concern | Yes (c-Met) | No | No | | Availability | Research chemical | Research chemical | Research chemical | | Safety Profile | Unknown | Well-established | Well-established |

Regulatory

Current Status

| Region | Status | |--------|--------| | United States | Not FDA-approved; unregulated research chemical | | WADA | Not specifically listed | | Clinical Trials | Not in active trials | | Patent Status | Patented (Washington State University) |

Legal Considerations

  • Not controlled substance
  • Available as research chemical
  • No regulatory oversight
  • Buyer assumes all risk
  • Quality varies dramatically

Scientific Outlook

Dihexa represents:

  • Fascinating preclinical research
  • Potential for dementia/cognitive treatment
  • Needs clinical trial validation
  • Cancer safety concerns must be addressed
  • Currently too experimental for recommendation

References

[1] Benoist CC, et al.. Dihexa: A small molecule that enhances cognition by increasing the efficacy of NGF-induced TrkA signaling. Neuropharmacology (2014)
[2] McCoy AT, et al.. Evaluation of metabolically stabilized angiotensin IV analogs as procognitive/antidementia agents. Journal of Pharmacology and Experimental Therapeutics (2013)
[3] Wright JW, et al.. The brain hepatocyte growth factor/c-Met receptor system: a new target for the treatment of Alzheimer's disease. Journal of Alzheimer's Disease (2016)
[4] Cognitive Peptide Review. HGF/c-Met Activators as Cognitive Enhancers: Promise and Concern. Neuropharmacology Reviews (2025)