Solvent Guide

Understanding reconstitution solvents and proper preparation techniques.

For Research Purposes Only. This information is educational. Always use sterile technique and consult appropriate resources for your specific application.

Bacteriostatic Water

Sterile water containing 0.9% benzyl alcohol as a preservative. The most commonly used solvent for peptide reconstitution.

Common Uses

  • Most peptides
  • Multi-dose vials
  • Storage up to 28 days

Preparation

Ready to use - do not dilute

Storage

Room temperature, protect from light

Note: Safe for multiple withdrawals due to preservative. Most versatile option.

Sterile Water

Pure sterile water without preservatives. Used when bacteriostatic water is contraindicated.

Common Uses

  • Single-use applications
  • Immediate injection
  • When benzyl alcohol sensitivity exists

Preparation

Ready to use - do not dilute

Storage

Use immediately after opening

Note: No preservative means single-use only. Risk of contamination with multiple withdrawals.

Acetic Acid (0.6%)

Dilute acetic acid solution used for peptides that require lower pH for stability.

Common Uses

  • IGF-1 LR3
  • Some growth factors
  • Acid-stable peptides

Preparation

Mix 0.6mL glacial acetic acid with 99.4mL sterile water

Storage

Room temperature, tightly sealed

Note: Required for certain peptides. Check specific peptide requirements before use.

Sodium Chloride 0.9%

Normal saline solution. Isotonic with body fluids.

Common Uses

  • Dilution of concentrated peptides
  • Some clinical applications
  • IV preparations

Preparation

Ready to use or reconstitute from powder

Storage

Room temperature

Note: Isotonic - matches body fluid concentration. Good for larger volume injections.

Reconstitution Best Practices

  1. 1

    Prepare Your Workspace

    Clean your work area. Have all materials ready: vial, solvent, syringe, alcohol wipes.

  2. 2

    Clean Vial Tops

    Wipe the rubber stopper of both vials with an alcohol swab. Allow to dry completely.

  3. 3

    Draw Solvent

    Draw the desired amount of solvent into a sterile syringe. Refer to the dosing calculator for appropriate volumes.

  4. 4

    Add Solvent Slowly

    Inject the solvent slowly down the inside wall of the peptide vial. Do NOT spray directly onto the peptide powder - this can damage the peptide.

  5. 5

    Allow to Dissolve

    Let the vial sit and allow the peptide to dissolve naturally. You may gently swirl (not shake) to help dissolution. Wait until completely clear.

  6. 6

    Store Properly

    Most reconstituted peptides should be stored in the refrigerator (2-8C / 36-46F). Protect from light. Use within the recommended timeframe.

Storage Guidelines

Lyophilized (Powder)

  • Freezer (-20C): 12+ months
  • Refrigerator (2-8C): 3-6 months
  • Room temp: 1-3 months

Freeze for long-term storage. Protect from moisture.

Reconstituted (Liquid)

  • With BAC water: 21-28 days
  • With sterile water: Use immediately
  • Frozen: Not recommended

Always refrigerate. Do not freeze reconstituted peptides.

Need Help with Calculations?

Use our dosing calculator to determine the correct reconstitution volume and injection dose for your research.

Open Calculator